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Cook Flexipet

Cook® Flexipet® Adjustable Handle Cook Medica

The Flexipet® Denuding Pipettes are polycarbonate tubes with inner diameters measuring 600 µm (microns) at the proximal end which taper in diameter in a range from 120 µm to 170 µm on the distal.. Cook ® Fistula Brush J-FB-100 G48527 Brushes Colorectal Surgery, General Surgery, Obstetrics Surgery Cook ® Flexipet ® Adjustable Handle K-MPH-1000, K-FRO-1000 G18674, G18904 Accessories, Needles - Amniocentesis Cook® Flexipet® Pipettes CONSTITUENTS Calcium lactate D-glucose Glutamine, stabilized Glycine HEPES Human serum albumin (HSA)* L-alanine L-asparagine L-aspartic acid L-glutamic acid L-proline L-serine Magnesium sulphate Potassium chloride Potassium phosphate Purified water Sodium bicarbonate Sodium chlorid Flexipet Denuding Pipette, Flexipet Manipulation Pipette: Applicant: COOK INCORPORATED: 750 DANIELS WAY: bloomington, IN 47404 Applicant Contact: naomi funkhouser: Correspondent: COOK INCORPORATED: 750 DANIELS WAY: bloomington, IN 47404 Correspondent Contact: naomi funkhouser.

Cook ® Flexipet ® Adjustable Handle K-MPH-1000, K-FRO-1000 G18674, G18904 Accessories, Needles - Amniocentesis, Needles - Aspiration, Needles - Fetal Access Obstetrics Reproductive Health Cook ® Flexipet ® Pipett Cook® Flexipet® Manipulation Pipette 300 µm Inner Dimension Without Graduations Sterile Flexible polycarbonate pipettes used for denuding as well as gamete an Pipette containers are color coded according to size 5 containers of 10 manipulating pipette Cook® Flexipet® Manipulation Pipette 300 µm Inner Dimension Without Graduations Sterile. Flexible polycarbonate pipettes used for denuding as well as gamete an Pipette containers are color coded according to size 5 containers of 10 manipulating pipette Cook® Flexipet® Manipulation Pipette 300 µm Inner Dimension Without Graduations Sterile PIPETTE MANIPULATION (5/BX

Cook® Flexipet® Adjustable Handle (K-MPH-1000) Zpět na výpis produktů. Katalogové číslo: K-MPH-1000. Držák pro Flexipipety. \. · Používá se s Cook® Flexipipetami pro manipulaci gamet a embryí. · Nastavitelná rukojeť akceptuje všechny velikosti Flexipipet. · Rozmezí aspiračního objemu je 0.25 µl až 3.0 µl. - Poskytuje. Cook® Flexipets® Pipettes. K-FPIP-1600-10BS-5. Cook® Flexipets® Pipettes. K-FRO-1000. Cook® Flexipet® Adjustable Handle - Accessories. K-HPIP-1030. Cook® Precision Holding Pipettes. K-HPIP-1035. Cook® Precision Holding Pipettes Cook Flexipet Pipettek Medical. Despriction: Cook&Flexipet&Pipette Cook Medical. 18 rowsCook&Flexipet&Pipette Flexible polycarbonate pipettes used for denuding as well as gamete and embryo manipulation.. The flexible polycarbonate will not break or scratch dishes. The pipette containers are color coded according to size used with cook Flexipets for handling gametes and embryos. aspiration volume can be preset from 0.25 μl to 3.0 μl. provides the fine control necessary for oocyte, embryo and/or blastomere manipulation. adjustable handle accepts all sizes of Flexipets. accessory set consists of replacement parts including one small o-ring, one large o-ring and a spacer

COOK Flexipet Pipettes/Denuding Pipettes COOK Flexipet Adjustable Handle COOK Fleximate COOK Micromanipulation Pipettes Clinical Laser Assisted Hatching Oosight Imaging System The COOK Insemi-Cath is used for the introduction of washed spermatozoa into the uterine cavity.. Cook sells directly to hospitals and physicians and also through selected fi rms; contact the offi ces listed below for sales and product information in your area. COOK IRELAND LTD. O'Halloran Road National Technology Park Limerick, IRELAND Phone: +353 61 334440 Fax: +353 61 334441 COOK MEDICAL INCORPORATED P.O. Box 4195 Bloomington, IN.

COOK Flexipet Adjustable Handle COOK Fleximate COOK Micromanipulation Pipettes Clinical Laser Assisted Hatching Oosight Imaging System Embryo Culture Incubators COOK Culture Media Oosafe Plasticware Camera Witness Embryo Transfer Catheters Lab Air Quality & VOC Control. Flexipet Denuding pipette 130 micron. The product is boxed with 5 vials of 10. The device is used for the intracytoplasmic single sperm injection of oocytes. Code Information: Lot number N74091: Recalling Firm/ Manufacturer: Cook Vascular Inc. 1186 Montgomery Ln Vandergrift PA 15690-6065: For Additional Information Contact: Thomas Kardos 724. A Cook FlexipetTM (Cook Medical) was used to pick up the follicles and transfer them 95 into a 1.5mL Eppendorf tube with 250 uL of 10% FBS at 4oC. 96 97 For cryopreserved tissue digestion, the cortical tissue became less pliable and the follicles less 98 prominent from the surrounding stromal tissue, so two modifications were made to aid with. Without the actual device, a root cause for the reported issue (some ¿oil¿ in the front part of product) cannot be determined. Per the quality engineering risk assessment, no further action is required. Cook medical has notified the appropriate personnel and will continue to monitor this device via the complaints database for similar complaints Thank You for Your Interest in Cook Medical's Products. The product information on this website is intended only for physicians and healthcare professionals licensed in the European Union, the United Kingdom, Switzerland, Norway, Iceland, Turkey, and Liechtenstein. If you are located in another global region, please click on the regional flag.

You will be leaving the Cook Medical website that you were viewing and going to a Cook Medical website for another region or country. Not all products are approved in all regulatory jurisdictions. The product information on these websites is intended only for licensed physicians and healthcare professionals We are a Medical Devices distribution company, offering various solutions in the Healthcare Industry for the last 21 years and are opening multiple offices PAN India. InterMedics is the authorized all India distributor for various big international companies like Cook Medical, Teleflex Medical, BARD, Hamilton Thorne etc Flexipet Pipettes by Cook Medical. Designed for use in reproductive health; Made of flexible polycarbonate that resists breaking and scratching dishes; Pipette containers are color coded according to size for quick identification; COKG26712 denuding pipette has a 140 micrometer I.D G26712 Denuding Pipette Cook® Flexipet® 140 µm Inner Dimension Without Graduations Sterile PIPETTE, FLEXIPET DENUDING (5 /BX) Add to quote G22761 PEG Pull Kit Flow 20® 20 Fr. 150 cm Silicone Sterile GASTROSTOMY SET, ENDO PERCUTANEQUS FLOW 20 PUL

Cook Medical G26712 - McKesson Medical-Surgica

  1. MICROCISE FLEXIMATE TOOL FOR HANDLING AND DENUDING FLEXIPET: Product Code: K-FMDT-1000: Manufacturer of the medical device: COOK INCORPORATED: Category Name: ASSISTED REPRODUCTION DEVICES - OTHERS: Category Code: U080299: Sign repertoire: Not Registered: Group of similar Medical devices: 0: Medical device reference: 812107: Type: Medical Device.
  2. ORIGIO® Micropipettes In 1992 the first commercial high quality micropipets for IVF professionals was marketed by HUMAGEN. Product development of the micropipets has always been based on customers' needs and requirements. HUMAGEN micropipets are now marketed as ORIGIO micropipets - the quality and consistency of the products remain at the same high level.The range o
  3. gton, IN, USA) or pulled glass micropipettes of 140- and 300-µm inner diameter. Animal Experiments In order to study the development of teratomas and teratocarcinomas, we used as recipients immunodeficient nude mice (Nu/Nu) and immunocompetent C57Bl/6 mic
  4. COOK Medical by ARID obchodní společnost, s.r.o. Okružní 25 638 00 Brno Tel.: 545 429 211 Fax: 545 429 22
  5. gton, IN, USA) or pulled glass micropipettes of 140- and 300.

Products Cook Medica

  1. Pobočky Flexipal a.s., Hřbitovní 1871/67, 741 01 Nový Jičín, Czech republic - Veronika Vichrová, +420 604 427 725, veronika.vichrova@flexipal.com Flexipal a.s., Ledce 324, 330 14 Ledce u Plzně, Czech republic - Zdeněk Růžička, +420 605 317 773, zdenek.ruzicka@flexipal.com Lid
  2. COOK Flexipet Pipettes/Denuding Pipettes COOK Flexipet Adjustable Handle COOK Fleximate COOK Micromanipulation Pipettes Clinical Laser Assisted Hatching Oosight Imaging System The COOK Insemi-Cath is used for the introduction of washed spermatozoa into the uterine cavity..
  3. e, stabilized Glycine HEPES Human serum albu

BD Vacutainer® Syringes for Arterial Blood Gas Analysis Drihep™ A-LINE, 3ml, Luer Lo All MII oocytes (n = 47,315) with a normal morphological appearance, both in vivo and in vitro matured, were inseminated by ICSI 3-4 h after egg collections.The total number of oocytes injected represented a 20.2% increase in the number of mature oocytes. Seventy-one point six percent of the metaphase I oocytes collected completed their nuclear maturation in vitro, in the time from oocyte. For the study group (closed system), the oocytes were washed in G1 media after injection, transferred with a Cook Flexipet (Cook, Limerick, Ireland) to EmbryoSlides prepared as described below, and cultured in the EmbryoScope™ until time of transfer About. DATUM is the single point of connection to automate the synchronization of accurate product information for all trading partners. TELUS DATUM is a web-based portal residing in a secure cloud environment that uses the GTIN as a single point of reference, and coordinates all other relevant information for that product, stores it within a safe database, and automatically transmits that. Cook® Flexipets® Pipettes : K-FPIP-1300-10BS-5: Cook® Flexipets® Pipettes : K-FPIP-1600-10BS-5: Cook® Flexipets® Pipettes : K-FRO-1000: Cook® Flexipet® Adjustable Handle - Accessories : K-HPIP-1030: Cook® Precision Holding Pipettes: K-HPIP-1035: Cook® Precision Holding Pipettes: K-HPIP-2130: Cook® Precision Holding Pipettes: K-HPIP-213

The mitochondrial suspension was further concentrated by transferring it to a sealed straw (Flexipet, Cook Australia) followed by centrifugation for 25 seconds at 10,000 g. The mitochondrial. ©Medline Industries, Inc. All rights reserved. Advancing the Health of Healthcare is a trademark and Medline is a registered trademark of Medline Industries, Inc Cook® Flexipet® Adjustable Handle Used with Flexipet denuding and manipulation pipettes. • Aspiration volume that can be preset from 0.25 µL to 3.0 µL. • Provides the fine control necessary for oocyte, embryo, and/or blastomere manipulation. • Adjustable handle that accepts all sizes of Flexipet pipettes. Order Number Reference Part. Flexipet Manipulation Pipette 300 micron. The product is boxed with 1 vial of 10 pipettes. This product is used for the intracytoplasmic single sperm injection of oocytes. Code Information: Lot number 72169: Recalling Firm/ Manufacturer: Cook Vascular Inc. 1186 Montgomery Ln Vandergrift PA 15690-6065: For Additional Information Contact: Thomas.

Vacuum-based micromanipulation pipettes can be used as alternatives to mouth pipetting (Flexipet, Cook or transfer pipette, Vitrolife) REAGENT SETUP PMSG Niryat Bhawan, Rao Tula Ram Marg, Opp. Army Hospital Research & Referral, New Delhi -110057, INDIA Phone: 91-11-26150101-04/46042222 Fax: 91-11-26150066/26150077 Email: fieo@fieo.or Scope Medical Company 80 Mohi El Din Abou El Ezz Mohandessin, Cairo, Egypt Tel : (+202) 37601844 - 3760651 Flexipet for thawing blastocyst-stage embryos (300 μm; Cook, cat. no. K-FPIP-1300-10BS) Holding micromanipulation pipette (Humagen, cat. no. MPH-MED-35) Sampling micromanipulation pipette. Then, ovaries were placed in 30 μl isolation media and prenatal follicles were dissected mechanically with a 29-gauge needle. Two hundred fifty six morphologically healthy follicles were picked up with 130-μm micropipette (Flexipet; Cook) . Follicles were picked up within 45 min under a stereomicroscope (Leica; Van Hopplynus Instruments)

cook drainage pouch set (500 cc) cook drainage pouch single 500cc (sterile) cook emergency airway bah . cook emergency pneumothorax set . cook enforcer balloon catheter . cook enforcer balloon catheter 18fr . cook flexible biopsy forceps . cook gianturco-rosch covered esophageal extension . cook guided bone biopsy needle set . cook. Cumulus-corona-oocyte (CCO) complexes denudation was carried out using hyaluronidase SynVitro ® Hyadase; Origio ® Solution, Màlov, Denmark) for a maximum of 30 sec with a denudation pipette (Denudation pipette Flexipet ®, Cook ® Medical, Bloomington, IN, USA). Removal of cumulus-corona cells is required to evaluate and classify the oocyte. zygotes, the Cook-IVF-Flexipet denuding glass pipette (170 mm) was used with 2 ml of solution (Liebermann et al., 2002b). In the present report, we assessed the survival rate of excess human compacted morulae and early blastocysts that were experimentally vitrified by the OPS method using a plasti COOK Medical. Endoscopic Injection Needle. Fallopian Tube Catheterization Set. Flexipet Adjustable Handle. FluoroSet Radiographic Tubal Assessment Set. Goldstein SonoBiyopsi Kateteri. Harrison Fetal Bladder Stent Set. Hyaluronidaz K-SIHY-1-5. ICSI Holding Pipette. Immature Ovum Aspiration Needle Set Cook Flexipet nastavitelný držák. Cook silikonový balónkový HSG katetr. Děložní balónkový stent. Bush DL ureterální světelný katetr. Vakuová Pumpa - Aspirační Jednotka. Test Tube Heater. UMI-Lock děložní manipulátor a injektor. Tao Brush endometriální kartáček

510(k) Premarket Notificatio

  1. The proportions of values above the LOQ (0.5 ng/ml) between the three brands of strippers were not statistically different (7/20 for Cook, 10/20 for Origio and 6/20 for Research Instruments strippers; P = 0.4, Chi-squared test). The proportions of values above the LOQ were not significantly different (Fisher exact test) between the first five.
  2. Main outcomes: Clinical pregnancy. Results: 60,955 oocytes were collected from the 3135 ICSI cycles, 57,214 (93.9%) were normal and 39,364 (68.8%) of these were metaphase II oocytes. 71.6% of.
  3. , with exposure to VS1 for 4
  4. COOK Medical Flexipet Instructions For Use Manual User manual manual (32 pages) Cole-Parmer Stir-Pak 04555-00 Operating Manual Operating manual (8 pages) Motorola LI4278 Quick Start Manual Quick start manual (2 pages) Agilent Technologies 7890B Operation Manual Operation manual (196 pages
  5. Heidolph Titramax 101 Manuals & User Guides. User Manuals, Guides and Specifications for your Heidolph Titramax 101 Laboratory Equipment. Database contains 2 Heidolph Titramax 101 Manuals (available for free online viewing or downloading in PDF): Operating manual, Instruction manual

The Apex export promotion organisation of India. Premier institution for promoting India's international business. The Government of India is partner organisation in the national export effort. The Indian private sector's effort to expand and extend its global business reach. An expression of the global business aspiration of 10,000 and more private exporters of India using a 140 m Flexipet (Cook Australia Pty Ltd., Brisbane, Australia) in combination with pharmaceutical grade hyaluronidase (Hyalasefi; Sanofi-Aventis Australia Pty Ltd., Brisbane, Australia) reconstituted in pH-stabilised G-MOPS media (Vitrolife, Gothenburg, Sweden). GV oocytes wer Using a stereomicroscope, leukemic cells from both cell lines were retrieved with a 130-μm micropipette (Flexipet, Cook) and placed in separate medium droplets. Incorporation of cells into a fibrin matrix. Fibrin clots were formed as previously described by Luyckx et al. . A 12.5-μL fibrinogen droplet (Baxter, Belgium) was placed on a glass. reusable handle for pipettes flexipet (tm) roadrunner's guide ® pc with wool jacket slipcoat; set hsg tubal; set insemination; set insemination; set insemination; set to cook venography pelvic transcervical; set with catheter balloon in silicon for word bartholin's gland; set with catheter for transfer of intratubal gametes by laparoscopi A Flexipet denuding pipette (Cook), with an inner diameter of 120 μm attached to a Flexipet Adjustable Handle Set was used to create microdrops under oil. The instrument can deliver a volume of 0.25-3.0 μL. The pipette was rinsed in cleavage medium before use. The transfer was done by moving the embryo in and out at the very tip of the pipette

Cook Medical G26713 - McKesson Medical-Surgica

>Medicīnas iekārtas > Mākslīgā apaugļošana > Mikromanipulāciju pipetes un fleksiblās pipetes > Cook Medical IVF mikromanipulāciju pipete flexipet denuding pipet 1401.1m cook k-fpip-1140-10bs-5 flexipet denuding pipet 3001.1m cook k-fpip-1300-10bs-5 tips 100 microliters single sterile eppendorf cannula 'ui memory ccd laboratoire 12040mf catheter embryo transfer soft with guide frydman ccd 130700 through 170 lm internal diameter pipettes (Flexipet; Cook, Bloomington, IN, USA) after a brief exposure to 40 IU/ml of hyaluronidase (Sage) in IVF medium (Sage). A maximum of three metaphase II (MII) fresh oocytes were destined to rou-tine ICSI procedure and subsequent embryo transfer. Patients were included in the study only when a cohort o the help of polycarbonate pipettes (Flexipet; Cook Spain, Madrid, Spain). The oocytes were checked for the presence of the first polar body in the perivitelline space (indicating that the oocytes were in metaphase II) and the morphological appearance of the gamete was evaluated (size of the perivitelline space, cell membrane and ooplasm) (Flexipet, Cook, USA). Ejaculated sperm samples were processed for ICSI by using discontinuous two-layer density gradient or simple washing. Percutaneous A single medium and sequential media are equally effective for the culture of human embryos to the blastocyst stage: a pilot study INTRODUCTION Several culture media have been designed fo

Treatment of human sperm with serine protease during density gradient centrifugation J. Fourie1, N. Loskutoff2 and C. Huyser1 1Department of Obstetrics & Gynaecology, Steve Biko Academic Hospital, University of Pretoria, Gauteng, South Africa 2The Bill & Berniece Grewcock Center for Conservation & Research, Omaha, NE, USA Correspondence J. M. Fourie, Department of Obstetrics and Gynaecology. Mechanical denudation was carried out in enzyme-free HEPES-buffered medium (50 μL droplets) by repeated aspiration into stripper tips (Flexipet, Cook Medical, Bloomington, IN, USA). All metaphase II oocytes were injected using a 35-degree angled ICSI pipette (Cook Medical, Bloomington, IN, USA) (Flexipet) (Cook, Bloomington, IN, USA) in a controlled CO2 and temperature environment. Oocyte vitrification was carried out after nuclear maturity assessment under stereomicroscope. Only MII oocytes were eligible for vitrification. Vitrification protocol The RapidVit Kit® (Vitrolife, Göteborg, Sweden) and the closed syste

After an incubation of 2 ± 0.5 h at 37°C in an atmosphere of 5.5% CO 2 in air, cumulus cells were completely removed from all oocytes by pipetting them through 170 μm internal diameter pipettes (Flexipet; Cook, Bloomington, IN, USA) after a brief exposure to 40 IU/ml of hyaluronidase (Sage) in IVF medium (Sage). A maximum of three metaphase. 1. 042823948. NA. Cook Spectrum Turbo-Ject PICC Set Minocycline/Rifampin Impregnated Power Injectable Polyurethane Peripherally Inserted Central Venous Catheter. Cook Spectrum. 2. In Commercial Distribution. C-UTLMY-701J-ABRM-CUSTOM-0057. 00827002571655 follicles were recovered using a 130 μm micropipette (Flexipet; Cook, Ireland). They were then rinsed three times in phosphate-buffered saline (PBS) supplemented with 10% fetal bovine serum (FBS) (Sigma, St Louis, MO, USA) to remove the stromal cells. The follicular population was not characterize Over the course of one hour, isolated follicles were picked up by two operators using a 130-µm micropipette (Flexipet, Cook, Limerick, Ireland) and divided into two different groups, primordial-primary or secondary (Fig. 1), on the basis of their size After removing the needle, a Flexipet denuding pipette (120 µm inner diameter), attached to a Flexipet Adjustable Handle Set (Cook, Brisbane, Australia), loaded with seven blastocysts, was inserted 5 mm into the uterine horn and the blastocysts were gently expelled with minimal amount of culture medium

Cook Medical G46017 - McKesson Medical-Surgica

  1. ative vesicle stage), metaphase I (oocytes showing no ger
  2. gton, Ind. The FLEXIPET® pipette is formed from polycarbonate, and is generally capable of aspirating volumes from about 0.25 to 3.0 μl
  3. After an incubation period of 16 h at 37 °C, the hemizonae were transferred to freshly prepared micro droplets. Loosely bound sperm were removed by pipetting 4 times using a 130 μm pipette tip (Flexipet®; Cook). The numbers of tightly bound sperm were double-blind counted by two evaluators at 400 times magnification (Axiovert 200; Zeiss)
  4. For ICSI, 2 hours after oocyte collection, oocytes were stripped of the cumulus cells after treatment with hyaluronidase by aspirating several times through a 130-μm pipette (Flexipet, Cook). Oocytes showing first polar body extrusion were injected with a single spermatozoon as described by Van Steirteghem et al. . Injected oocytes were washed.

Cook Medical G46020 - McKesson Medical-Surgica

(Flexipet, Cook). Oocytes showing first polar body extrusion were injected with a single spermatozoon as described by Van Steirteghem et al. (23). Injected oocytes were washed and transferred to 10 L droplets of fertilization medium. All incubations were performed at 37°C in a humidified atmosphere of 5% O2,6%CO2, and 89% N2. Culture wa COCs were placed in the well with SynVitro Hyadase and gently aspirated up and down by a denuding pipette (Flexipet ® denuding pipette, COOK Medical, Bloomington, IN, USA). Oocytes were transferred into a well rinsed with culture medium and the remaining cumulus cells were removed Used with Flexipet denuding and manipulation pipettes. The aspiration volume can be preset from 0.25 μL to 3.0 μL. The handle provides the fine control necessary for oocyte, embryo, and/or blastomere manipulation. The handle is adjustable to accept all sizes of Flexipet pipettes

Cook® Flexipet® Adjustable Handle (K-MPH-1000) ARID s

Then, using a 135 μm diameter pipette (Flexipet, Cook, Bloomington, IN, USA), the oocytes were transferred to M-HTF for complete removal of the corona cells. The denuded oocytes were then rinsed and incubated in M-HTF until the time of injection (2-3 h after collection). The ICSI procedure was performed only for metaphase II (MII) oocytes. 2 mL was done using the Flexipet pipette in a similar way, except that 2 embryos were transferred at a time. Culture condition and assessment of embryo development Embryos were cultured in MINC Benchtop Incubators (K-MINC-1000; Cook)underanatmosphereof6%CO 2,5%O 2,and89%N 2 at37 C.Embryo developmentwasassessedunderan invertedmicroscopeafter24. 130-mm micropipette (Flexipet; Cook) over the course of 45 minutes (9, 10). Thereafter, the rest of the cell suspension was successively filtered through sterilized 80-mm and 11-mm nylon net filters (Millipore). OCs were counted with the use of trypan blue (Sigma-Aldrich) and a B€urker chamber (VWR) Flexipet oocyte/embryo pipettes (Cook Medical K-FPIP-1130-10BS-5). Tuberculin syringes. Holding pipettes. (Humagen MPH-SM-20). Chamber slide (Lab-Tek #177372). Equipment . Glass pipette puller: Sutter Instrument Co. Model -P97 outfitted with 2.5mm × 2.5mm Box filament (FB255B An alternative to mouth-controlled pipetting is the use of denuding and manipulation pipettes, as used in laboratories working in the field of reproductive medicine, i.e., the Flexipet pipette.

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Cook® Flexipets® Pipettes (K-FPIP-1300-10BS-5) ARID s

  1. gentle aspiration in and out of a plastic pipette (Flexipet, 140 μm, ID, Cook, Australia) (Rienzi et al., 1998). The denuded oocytes were then evaluated to asses their nuclear maturation stage. Only supernumerary oocytes at metaphase II MII stage were included in this study. Immediately after denudation, th
  2. Experimental vitrification of human compacted morulae and early blastocysts using fine diameter plastic micropipette
  3. The blastomere was then placed onto the microscope slide in a small hypotonic drop using 80 μ flexipet (Cook, Australia). A drop of chilled methanol: acetic acid (3:1) fixative was added over the blastomere, which resulted in bursting of the cytoplasm and fixation of blastomere nucleus on the slide
  4. The oocyte was isolated using a 140 μm pipette tip (Flexipet, Cook Women's Health, Spencer, IN). If the oocyte retained adherent cumulus cells, it was briefly (30 s) treated in a 100 microliter drop of 1% hyaluronidase (Sigma Chemical Co., St. Louis, MO) in TALP-Hepes with 0.3% BSA, to facilitate assessment of nuclear maturation [ 15 ]

Cook Flexipet Pipettek Medical - HDPE Water Supply Pipes

Using a stereomicroscope, 10 and 100 leukemic cells were retrieved with a 130 μm micropipette (Flexipet, COOK) and placed in separate medium droplets. Incorporation of cells into a fibrin matrix. Formation of fibrin clots was done as previously described by Luyckx et al. . A 12.5 μL fibrinogen droplet (Baxter, Belgium) was placed on a glass. Human oocytes were denuded of surrounding cumulus cells by means of mechanical pipetting using a 140 µm Flexipet (Cook Australia Pty Ltd., Brisbane, Australia) in combination with pharmaceutical grade hyaluronidase (Hyalase ®; Sanofi-Aventis Australia Pty Ltd., Brisbane, Australia) reconstituted in pH-stabilised G-MOPS media (Vitrolife.

Welcome to interMedics,Intrauterine Insemination(IUI

Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product-free environmen Two hours after oocyte retrieval, cumulus oophorus was denuded by a brief exposure to 80 IU/ml of hyaluronidase solution (hyaluronidase) (Fertipro™, Beernem, Belgium) followed by mechanical removal of the corona radiata with a denudation pipette (Flexipet) (Cook, Bloomington, IN, USA) in a controlled CO2 and temperature environment 300mm ID flexible, sterile denuding pipette (Flexipet®, COOK ® ) to load VTF solutions with oocytes or embryos before placing the VTF tip into a 0.3ml CBS TM embry For ICSI, 2 hours after oocyte collection, oocytes were stripped of the cumulus cells after treatment with hyaluronidase by aspirating several times through a 130-μm pipette (Flexipet, Cook). Oocytes showing first polar body extrusion were injected with a single spermatozoon as described by Van Steirteghem et al Times between OPU and ICSI (OPU-ICSI) ranged from 1 h 25 min to 17 h 13 min (average fresh ± SD = 4 h 58 m ± 1 h; average vitrified = 9 h 18 m ± 2 h). We found no effect of OPU-ICSI time on fertilization rate (p fresh =0.39; p vitrified =0.86) or embryo quality at Days 2 and 3 (p fresh =0.08; p vitrified =0.22).There was no difference in average OPU-ICSI times between positive and.

Class 2 Device Recall Flexipet Denuding pipette 130 micro

Insemination with standard IVF or ICSI was performed after an incubation of 3 ± 0.5 h in one of the two culture systems; cumulus cells were completely removed from all oocytes of ICSI cycles by pipetting them through 140/170 μm internal diameter pipettes (Flexipet; Cook, Bloomington, IN, USA) after a brief exposure to 40 IU/ml of. GV oocytes ruptured from ovary were released in M2 medium (Sigma, St. Louis, MO, USA) and cumulus cells were denuded through hyaluronidase digestion (Sigma). Oocytes at GV stage with similar size and morphology were selected mechanically by a FLEXIPET manipulation pipette (Cook Medical, Bloomington, IN, USA)

MAUDE Adverse Event Report: COOK INC FLEXI-PET ADJUSTABLE

A few microliters of the BV-173 cell suspension were added to a drop of fresh medium in a plastic petri dish. Using a stereomicroscope, 10 and 100 leukemic cells were retrieved with a 130 μm micropipette (Flexipet, COOK) and placed in separate medium droplets

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